Cloning and functional study of <i>GmRPI2</i>, which is the critical gene of photosynthesis in soybean

Light provides energy for photosynthesis and is also an important environmental signal that regulates plant growth development. Ribose-5-phosphate isomerase plays a crucial role in photosynthesis. However, ribose-5-phosphate has yet to be studied soybean To understand the biological function of GmRPI2, this study, GmRPI2 was cloned, overexpression vectors gene editing were successfully constructed, transformed into recipient JN74 using Agrobacterium-mediated method. Using qRT-PCR, we analyzed expression highest leaves, second roots, lowest stems. Promoter analysis revealed presence multiple cis-acting elements related light response promoter region GmRPI2. Compared with control plants, net photosynthetic rate transpiration lines higher than those lines, while intercellular CO2 concentration significantly lower lines; total chlorophyll, chlorophyll a, b contents soluble sugar plants indicating can increase The capacity providing theoretical basis genetic resources improving yield by regulating efficiency.